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KMID : 0613820090190111617
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Journal of Life Science
2009 Volume.19 No. 11 p.1617 ~ p.1622
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Optimun Conditions for Production of Mycelia and Extracellular Polysaccharide from Inonotus obliquus and Their Immunomodulating Acitivities
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Park Hee-Sung
Shin Dong-Il
Chung Il-Kyung
Yang Byung-Keun
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Abstract
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Optium conditions for the production of mycelia and extracellular polysaccharide (EXPS) from submerged mycelial culture of Inonotus obliquus and their immunomodulating activities were investigated. The optmium production of mycelia and EXPS from Ⅰ. obliquus was observed in mushroom complete medium (MCM). The optimum pH, temperature, and agitation speed for the production of mycelia and EXPS were 5.5, 25℃, and 150 rpm, respectively. The culture period for maximum production of mycelia (10.89 g/ℓ) and EXPS (1.25 g/ℓ) in shake flask cultivation was 11 days. The anticomplementary activity of intracellular polysaccharide (INPS) and EXPS form Ⅰ. obliquus increased in a dose-dependent manner. Lysosomal enzyme activity of EXPS and INPS increased by 2.0- and 2.2-fold at 100 ㎍/㎖ concentration, respectively, compared to the control group.
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KEYWORD
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Inonotus obliquus, extracellular polysaccharide, intracellular polysaccharide, anti-complementary activity, lysosomal enzyme activity
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